منابع مشابه
RNase YI* and RNA structure studies.
The enzymology of RNase YI*, a recently discovered endoribonuclease from yeast, was studied and compared to other endonucleases for detection of single-strand regions and base pair mismatches in RNA. Its value for RNA structure analyses was assessed with Escherichia coli 5S rRNA as a model substrate. The generally accepted structure of the 5S rRNA is based on thermodynamic energy considerations...
متن کاملRNase-assisted RNA chromatography.
RNA chromatography combined with mass spectrometry represents a widely used experimental approach to identify RNA-binding proteins that recognize specific RNA targets. An important drawback of most of these protocols is the high background due to direct or indirect nonspecific binding of cellular proteins to the beads. In many cases this can hamper the detection of individual proteins due to th...
متن کاملStudies on Methanocaldococcus jannaschii RNase P reveal insights into the roles of RNA and protein cofactors in RNase P catalysis
Ribonuclease P (RNase P), a ribonucleoprotein (RNP) complex required for tRNA maturation, comprises one essential RNA (RPR) and protein subunits (RPPs) numbering one in bacteria, and at least four in archaea and nine in eukarya. While the bacterial RPR is catalytically active in vitro, only select euryarchaeal and eukaryal RPRs are weakly active despite secondary structure similarity and conser...
متن کاملNew insight into RNase P RNA structure from comparative analysis of the archaeal RNA.
A detailed comparative analysis of archaeal RNase P RNA structure and a comparison of the resulting structural information with that of the bacterial RNA reveals that the archaeal RNase P RNAs are strikingly similar to those of Bacteria. The differences between the secondary structure models of archaeal and bacterial RNase P RNA have largely disappeared, and even variation in the sequence and s...
متن کاملRNase P RNA-mediated catalysis.
The endoribonuclease RNase P is involved in the processing of tRNA precursors to generate mature 5' termini. The catalytic activity of RNase P is associated with an RNA, RNase P RNA. A specific interaction between the 3' end of the substrate and RNase P RNA, to form an RNase P RNA-substrate complex, is referred to as the '73-294-interaction'. This interaction has an important role for efficient...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1997
ISSN: 1362-4962
DOI: 10.1093/nar/25.7.1405